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epifluorescence inverted microscope  (Nikon)


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    Structured Review

    Nikon epifluorescence inverted microscope
    Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epifluorescence+microscope/ECLIPSE+Ti2/pm41894427-71-8-11
    Average 99 stars, based on 11616 article reviews
    epifluorescence inverted microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Inverted Epifluorescence:

    Article Title: Event-based optical imaging and reconstruction of in vivo neuronal and vascular dynamics
    Article Snippet: .. Event camera optical setup The event camera (DVXplorer, iniVation) was mounted on the left port of an inverted epifluorescence microscope equipped with a motorized filter wheel (Eclipse Ti2, Nikon; Fig. 1). .. An sCMOS camera (Kinetix22, Teledyne Photometrics) was attached to the eyepiece port to enable simultaneous fluorescence imaging.

    Article Title: A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.
    Article Snippet: Two additional 5-min washes with 2× SSC buffer were performed, followed by rinsing with a wash buffer containing (25% formamide (Ambion, AM9342), 2× SSC, and nuclease-free water) and a final 5-min incubation in fresh wash buffer at RT. .. Probes were then diluted to 2 ng/mL in hybridization buffer (25% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906), 1 mg/mL E. coli tRNA (Sigma-Aldrich, R4251), 2× SSC, and 0.02% BSA (Ambio, AM2616)) and hybridized to tissues overnight at 37 ◦ C. Unbound probes were removed by washing with the same buffer for 30 min at 37 ◦ C. Nuclei were counterstained with 2 ng/mL DAPI (Sigma-Aldrich, D9564) for 5 min at 30 ◦ C. Finally, samples were mounted with Antifade Mounting Medium (Beyotime, P0126) and imaged using an inverted epifluorescence microscope (Nikon Eclipse Ti2). ..

    Article Title: N-acetyl- l -leucine lowers α -synuclein levels and improves synaptic function in Parkinson’s disease models
    Article Snippet: .. Cells were transferred to an imaging chamber equipped with field stimulation (RC-49MFSH, Warner Instruments) and mounted on an inverted epifluorescence microscope (Nikon TE300) with a 40×/1.35 NA oil-immersion objective. ..

    Article Title: Botulinum toxin-induced masseter muscle atrophy is associated with impaired autophagic flux without signs of apoptosis in mice
    Article Snippet: LC3 and SQSTM1/p62 images were acquired with a Carl Zeiss Axiovert 135 M-LSM Microsystems confocal microscope (CEMC, Faculty of Medicine, Universidad de Chile) and processed using the ImageJ program (Version 1.53k). .. Caveolin-3 images were acquired with an inverted epifluorescence microscope (Ti-E, Nikon®, USA) using the 20X objective, and the fiber diameter was quantified using Feter’s minimum diameter with the Dragonfly software (Version 2022.2.0.1409, Object Research Systems, Canada). .. The TUNEL Kit (DeadEndTM Fluorometric TUNEL System, Promega, G3250, WI, USA) was used on the samples mounted on the slide.

    Microscopy:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: .. The chamber was gently swirled to concentrate the neurospheres in the middle of the chamber, before being mounted onto an epifluorescence microscope (Nikon Elipse TiE) equipped with a 10X/0.4 NA dry objective, a mercury lamp (Nikon Xcite), and a filter set for Fluo-4 (Excitation: FF01-472/30; Dichroic: FF-495Di02; Emission: FF01-525/30). ..

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: Finally, neurospheres were collected and mounted in Fluoromount-G (SouthernBiotech, #0100-01) or RapiClear 1.52 (SUNJin Lab, #RC152001) between a 12 mm coverslip and a microscopy glass slide, using 150 μm thick spacers (SUNJin Lab, #IS015). .. Neurospheres from KI mice where endogenous bAP-NLGN1 was biotinylated with BirA ER AAV1s and further labeled with SA-AF647, were observed under an epifluorescence microscope (Nikon Elipse TiE) equipped with a 10X/0.4 NA dry objective with a mercury lamp (Nikon Xcite) and filter sets for EGFP (Excitation: FF01-472/30; Dichroic: FF-495Di02; Emission: FF01-525/30) and AF647 (Excitation: FF02-628/40; Dichroic: FF-660Di02; Emission: FF01-692/40) (SemROCK). .. Images of 100-500 ms exposure time were acquired with an sCMOS camera (Prime 95B, Photometrics, Tucson, USA) driven by the Metamorph software.

    Article Title: Reelin regulates the migration and differentiation of extravillous trophoblastic cells.
    Article Snippet: .. Using an epifluorescence microscope (Nikon Eclipse Ti-S) with a 20X objective and the NIS-Elements F program, bright-field images of the wound were taken in each well at 0 and 24 h, in the same position, using reference marks initially placed. .. For cell migration quantification at 24 h, the cellfree area was outlined and measured using the freehand selection tool in ImageJ software.

    Hybridization:

    Article Title: A tumor-resident bacterium promotes cervical cancer progression through lysoPC-mediated c-Jun/c-Fos activation.
    Article Snippet: Two additional 5-min washes with 2× SSC buffer were performed, followed by rinsing with a wash buffer containing (25% formamide (Ambion, AM9342), 2× SSC, and nuclease-free water) and a final 5-min incubation in fresh wash buffer at RT. .. Probes were then diluted to 2 ng/mL in hybridization buffer (25% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906), 1 mg/mL E. coli tRNA (Sigma-Aldrich, R4251), 2× SSC, and 0.02% BSA (Ambio, AM2616)) and hybridized to tissues overnight at 37 ◦ C. Unbound probes were removed by washing with the same buffer for 30 min at 37 ◦ C. Nuclei were counterstained with 2 ng/mL DAPI (Sigma-Aldrich, D9564) for 5 min at 30 ◦ C. Finally, samples were mounted with Antifade Mounting Medium (Beyotime, P0126) and imaged using an inverted epifluorescence microscope (Nikon Eclipse Ti2). ..

    Imaging:

    Article Title: N-acetyl- l -leucine lowers α -synuclein levels and improves synaptic function in Parkinson’s disease models
    Article Snippet: .. Cells were transferred to an imaging chamber equipped with field stimulation (RC-49MFSH, Warner Instruments) and mounted on an inverted epifluorescence microscope (Nikon TE300) with a 40×/1.35 NA oil-immersion objective. ..

    Labeling:

    Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses
    Article Snippet: Finally, neurospheres were collected and mounted in Fluoromount-G (SouthernBiotech, #0100-01) or RapiClear 1.52 (SUNJin Lab, #RC152001) between a 12 mm coverslip and a microscopy glass slide, using 150 μm thick spacers (SUNJin Lab, #IS015). .. Neurospheres from KI mice where endogenous bAP-NLGN1 was biotinylated with BirA ER AAV1s and further labeled with SA-AF647, were observed under an epifluorescence microscope (Nikon Elipse TiE) equipped with a 10X/0.4 NA dry objective with a mercury lamp (Nikon Xcite) and filter sets for EGFP (Excitation: FF01-472/30; Dichroic: FF-495Di02; Emission: FF01-525/30) and AF647 (Excitation: FF02-628/40; Dichroic: FF-660Di02; Emission: FF01-692/40) (SemROCK). .. Images of 100-500 ms exposure time were acquired with an sCMOS camera (Prime 95B, Photometrics, Tucson, USA) driven by the Metamorph software.

    Software:

    Article Title: Botulinum toxin-induced masseter muscle atrophy is associated with impaired autophagic flux without signs of apoptosis in mice
    Article Snippet: LC3 and SQSTM1/p62 images were acquired with a Carl Zeiss Axiovert 135 M-LSM Microsystems confocal microscope (CEMC, Faculty of Medicine, Universidad de Chile) and processed using the ImageJ program (Version 1.53k). .. Caveolin-3 images were acquired with an inverted epifluorescence microscope (Ti-E, Nikon®, USA) using the 20X objective, and the fiber diameter was quantified using Feter’s minimum diameter with the Dragonfly software (Version 2022.2.0.1409, Object Research Systems, Canada). .. The TUNEL Kit (DeadEndTM Fluorometric TUNEL System, Promega, G3250, WI, USA) was used on the samples mounted on the slide.



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